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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ELF4 / MEF Antibody (aa141‑190) LS‑C117277
MEF antibody LS-C117277 is an unconjugated rabbit polyclonal antibody to mouse MEF (ELF4). Validated for WB.
Catalog
Size
Price
LS-C117277-50
50 µg
Unavailable

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Product Description

MEF antibody LS-C117277 is an unconjugated rabbit polyclonal antibody to mouse MEF (ELF4). Validated for WB.
About ELF4 / MEF
Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Q99607 NM_001421 NP_001412.1

ELF4 Antibody, E74-like factor 4 Antibody, ELFR Antibody, MEF Antibody, Myeloid Elf-1-like factor Antibody


Specifications

Target
Mouse ELF4 / MEF
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ELF4 / MEF antibody was raised against synthetic peptide located between aa141-190 of mouse Elf4 (B1AY67, NP_062654). Percent identity by BLAST analysis: Mouse (100%).
Blocking Peptide
LS-E13359 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C117277. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa141-190
Specificity
Mouse ELF4
Usage
Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELF4 antibody LS-C117277 Western blot of NIH3T3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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