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Anti-EIF4G3 Antibody (clone 1H5) LS-C133563

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EIF4G3 Antibodies

Anti-EIF4G3 Antibody LS-C410234
Rabbit Polyclonal (IgG) to Human EIF4G3
Human, Mouse, Rat
IHC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1H5] (IgG2b,k) to Human EIF4G3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human EIF4G3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [1H5]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (3 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

EIF4G3 antibody was raised against eIF4G3 (AAH30578, 1 a.a. ~ 516 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human EIF4G3
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About EIF4G3

O43432 NM_003760 NP_003751.2

EIF4G3 Antibody, EIF-4-gamma II Antibody, EIF4G 3 Antibody, EIF-4-gamma 3 Antibody, EIF-4G 3 Antibody, EIF4GII Antibody

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 3 ug/ml].

Western blot

Western blot
Western blot of EIF4G3 expression in transfected 293T cell line by EIF4G3 monoclonal antibody (M03), clone 1H5.

Western blot

Western blot
EIF4G3 monoclonal antibody (M03), clone 1H5. Western blot of EIF4G3 expression in HeLa.

Western blot

Western blot
EIF4G3 monoclonal antibody (M03), clone 1H5 Western blot of EIF4G3 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged EIF4G3 is approximately 1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of EIF4G3 over-expressed 293 cell line, cotransfected with EIF4G3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF4G3 monoclonal antibody, clone 1H5. GAPDH ( 36.1 kD ) used as specific.

Requested From: United States
Date Requested: 12/9/2016

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