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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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EIF4ENIF1 / 4E‑T Antibody (aa215‑264) LS‑C112035
4E-T antibody LS-C112035 is an unconjugated rabbit polyclonal antibody to 4E-T (EIF4ENIF1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C112035-50
50 µg
Unavailable

Popular EIF4ENIF1 / 4E-T Products

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Product Description

4E-T antibody LS-C112035 is an unconjugated rabbit polyclonal antibody to 4E-T (EIF4ENIF1) from human, mouse, rat and other species. Validated for WB.
About EIF4ENIF1 / 4E-T
EIF4ENIF1 / 4E-T is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. NM_019843

EIF4ENIF1 Antibody, 4E-T Antibody, EIF4E transporter Antibody, EIF4E-transporter Antibody, Clast4 Antibody


Specifications

Target
Human EIF4ENIF1 / 4E-T
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Monkey, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa215-264 of human EIF4ENIF1 (Q9NRA8, NP_062817). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Guinea pig, Turkey, Chicken, Armadillo, Platypus, Xenopus (100%); Monkey, Horse (92%).
Blocking Peptide
LS-E20932 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112035. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa215-264
Specificity
Human EIF4ENIF1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF4ENIF1 antibody LS-C112035 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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