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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4EBP1 / 4EBP1 Antibody (phospho‑Thr45) LS‑C117284
Note: This antibody replaces LS-C63331, LS-C51216, LS-C34177
4EBP1 antibody LS-C117284 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C117284-50
50 µl
Unavailable
LS-C117284-100
100 µl
Unavailable

Popular EIF4EBP1 / 4EBP1 Products

Antibody
Species: Mouse, Human, Rat
Applications: Western blot, Immunoprecipitation, ELISA
IHC of paraffin-embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E-BP1 antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Mouse, Human, Monkey, Rat, Drosophila
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Species: Human, Mouse, Rat, Hamster, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Gel shift
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, Western blot, ELISA

Product Description

4EBP1 antibody LS-C117284 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About EIF4EBP1 / 4EBP1
EIF4EBP1 / 4EBP1 is one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. Q13541 NM_004095 NP_004086.1

EIF4EBP1 Antibody, 4E-BP1 Antibody, 4EBP1 Antibody, BP-1 Antibody, PHAS-I Antibody, EIF4E-binding protein 1 Antibody


Specifications

Target
Human EIF4EBP1 / 4EBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human 4E-BP1 around the phosphorylation site of Thr45.
Epitope
pThr45
Specificity
4E-BP1 (Phospho-Thr45) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine45.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from MDA-MB-435 cells treated with EGF 200 ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/20/2018

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