Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Bovine (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
Protein A purified
ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)
Specificity and Use
EIF3K antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 173 of EIF3K (SwissProt Q9UBQ5). Percent identity by BLAST analysis: Human, Mouse, Bovine (100%); Xenopus (93%).
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. EIF3K protein stained by EIF3K antibody [N1C2] diluted at 1:500.
Sample (30 ug of whole cell lysate). A: H1299. 12% SDS PAGE. EIF3K antibody diluted at 1:1000.
Requested From: United States
Date Requested: 7/27/2016