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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2S1 Antibody (Ser51) IHC‑plus™ LS‑B1469
Note: This antibody replaces LS-C34465
EIF2S1 antibody LS-B1469 is an unconjugated rabbit polyclonal antibody to EIF2S1 from human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1469-50
50 µg (1 mg/ml)
Unavailable

Popular EIF2S1 Products

EIF2S1 antibody LS-C31787 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
EIF2S1 antibody LS-C110529 Western blot of Fetal Small Intestine lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish, Drosophila
Applications: Western blot
Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6273 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-EIF2S1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7198 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

EIF2S1 antibody LS-B1469 is an unconjugated rabbit polyclonal antibody to EIF2S1 from human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About EIF2S1
EIF2 is a translation initiation factor that catalyzes the first regulated step of protein synthesis, promoting the binding of the initiator tRNA to 40s ribosomal subunits. eIF2 is composed of 3 non identical subunits, alpha, beta and gamma. Binding occurs as a ternary complex of methionyl-tRNA, eIF2 and GTP. The rate of complex formation is modulated by the phosphorylation state of eIF2alpha. Phosphorylation of eIF2alpha has been shown to occur in response to heat shock and hypoxic stress. P05198 NM_004094 NP_004085.1

EIF2S1 Antibody, EIF-2-alpha Antibody, EIF-2alpha Antibody, EIF2 alpha Antibody, EIF2 Antibody, EIF-2 Antibody, EIF-2A Antibody


Specifications

Target
Human EIF2S1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EIF2S1 antibody was raised against synthetic peptide from human EIF2S1.
Epitope
Ser51
Specificity
Amino acids surrounding Ser 51 of human EIF2A
Usage
Immunohistochemistry: LS-B1469 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1469 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% sodium azide, 50% glycerol.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.

Western blot

Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.
Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.

Western blot

Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.
Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.

Western blot

Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.
Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunohistochemistry

Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.
Anti-EIF2S1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1469 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using eIF2a(Ab-51) antibody.

Western blot

Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.
Western blot of extracts from 3T3, HeLa and C6 cells using eIF2a(Ab-51) antibody.


Requested From: 
Date Requested: 6/18/2018

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