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Anti-EIF2AK2 / PKR Antibody (aa519-550) LS-C101296

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

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Rabbit Polyclonal to Human EIF2AK2 / PKR
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human EIF2AK2 / PKR
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

Specificity and Use

LS-E9537 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101296. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa519-550
This PKR (PRKR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-550 amino acids from the C-terminal region of human PKR (PRKR).

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About EIF2AK2 / PKR

P19525 NM_002759 NP_002750.1

EIF2AK2 Antibody, p1/eIF-2A protein kinase Antibody, p68 kinase Antibody, PRKR Antibody, EIF-2A protein kinase 2 Antibody, p68 Antibody, Protein kinase RNA-activated Antibody, Tik Antibody

IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1).

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
The anti-PRKR antibody is used in Western blot to detect PRKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).

Western blot

Western blot
Western blot of EIF2AK2 (arrow) using PRKR Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2) (Origene Technologies).

Requested From: 
Date Requested: 4/23/2017

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