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Anti-EGR4 / EGR-4 Antibody (C-Terminus) IHC-plus™ LS-B1525

Note: This antibody replaces LS-C30232

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml Unavailable

Related Products

10 EGR4 / EGR-4 Antibodies

Most Popular EGR4 / EGR-4 Antibodies

Anti-EGR4 / EGR-4 Antibody (C-Terminus) IHC-plus™ LS-B1525
Rabbit Polyclonal (IgG) to Human EGR4 / EGR-4
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-EGR4 / EGR-4 Antibody LS-C402257
Rabbit Polyclonal (IgG) to Human EGR4 / EGR-4
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EGR4 / EGR-4 Antibody (C-Terminus, Biotin) LS-C436424
Rabbit Polyclonal (IgG) to Human EGR4 / EGR-4
Human, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
IHC, IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image
Anti-EGR4 / EGR-4 Antibody (C-Terminus, FITC) LS-C436426
Rabbit Polyclonal (IgG) to Human EGR4 / EGR-4
Human, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
IHC, IHC - Paraffin, Western blot
FITC Conjugated
Immunohistochemistry Image
Anti-EGR4 / EGR-4 Antibody (C-Terminus, HRP) LS-C436427
Rabbit Polyclonal (IgG) to Human EGR4 / EGR-4
Human, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
IHC, IHC - Paraffin, Western blot
HRP Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human EGR4 / EGR-4
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human EGR4 / EGR-4
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Drosophila (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.06 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

EGR4 / EGR-4 antibody was raised against synthetic peptide from C-Terminus of human EGR4 (Q05215, NP_001956). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken (100%); Opossum (92%); Medaka, Drosophila, Water flea (90%); Xenopus (84%); Tamarin, Panda, Bat, Platypus (81%).
LS-E13666 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1525. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human EGR4
Immunohistochemistry: LS-B1525 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1525 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EGR4 / EGR-4

Q05215 NM_001965 Q05215

EGR4 Antibody, AT133 Antibody, Early growth response 4 Antibody, NGFIC Antibody, NGFI-C Antibody, EGR-4 Antibody, PAT133 Antibody

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Immunohistochemistry

Immunohistochemistry
Anti-EGR4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1525 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
EGR4 antibody LS-B1525 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/23/2017

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