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Anti-EGR2 Antibody (aa239-251) IHC-plus™ LS-B3255
Note: This antibody replaces LS-C54757, LS-C45556Most Popular EGR2 Antibodies
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Details
| Human EGR2 | |
| Goat | |
| Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity) | |
| Mouse, Rat, Hamster (at least 90% immunogen sequence identity) | |
| Polyclonal | |
| Unconjugated | |
| Immunoaffinity purified | |
| Unmodified |
Applications
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- ELISA (1:64000)
Specificity and Use
| EGR2 antibody was raised against synthetic peptide HGTAGPDRKPFPC from an internal region of human EGR2 (NP_000390.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%); Mouse, Rat, Hamster, Elephant (92%). | ||
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| aa239-251 | ||
| Human EGR2. | ||
| Immunohistochemistry: LS-B3255 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3255 was determined to be 3.75 ug/ml. |
Packaging
| Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide | |
| Store at -20°C. Avoid freeze-thaw cycles. | |
| For research use only. |
About EGR2
P11161
NM_000399
NP_000390.2
EGR2 Antibody, CMT1D Antibody, CMT4E Antibody, Early growth response 2 Antibody, E3 SUMO-protein ligase EGR2 Antibody, EGR-2 Antibody, KROX20 Antibody, Krox-20 Antibody, AT591 Antibody, Zinc finger protein Krox-20 Antibody
EGR2 is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
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Requested From:
Date Requested: 8/19/2017
Date Requested: 8/19/2017
