Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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EGR2 antibody was raised against synthetic peptide HGTAGPDRKPFPC from an internal region of human EGR2 (NP_000390.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%); Mouse, Rat, Hamster, Elephant (92%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3255. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunohistochemistry: LS-B3255 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3255 was determined to be 3.75 ug/ml.
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.