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EGR2 Antibody (aa239‑251) IHC‑plus™ LS‑B3255
Note: This antibody replaces LS-C54757, LS-C45556
EGR2 antibody LS-B3255 is an unconjugated goat polyclonal antibody to EGR2 from human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
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Species:
Human, Monkey, Mouse, Bovine, Horse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Product Description
EGR2 antibody LS-B3255 is an unconjugated goat polyclonal antibody to EGR2 from human, bovine, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About EGR2
EGR2 is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
P11161
NM_000399
NP_000390.2
EGR2 Antibody, CMT1D Antibody, CMT4E Antibody, Early growth response 2 Antibody, E3 SUMO-protein ligase EGR2 Antibody, EGR-2 Antibody, KROX20 Antibody, Krox-20 Antibody, AT591 Antibody, Zinc finger protein Krox-20 Antibody
Specifications
Target
Human EGR2
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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Immunogen
EGR2 antibody was raised against synthetic peptide HGTAGPDRKPFPC from an internal region of human EGR2 (NP_000390.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%); Mouse, Rat, Hamster, Elephant (92%).
Blocking Peptide
LS-E26387 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3255. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-B3255. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa239-251
Specificity
Human EGR2.
Usage
Immunohistochemistry: LS-B3255 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3255 was determined to be 3.75 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
Anti-EGR2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Immunohistochemistry
Anti-EGR2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Western blot
Antibody (0.03 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry
Anti-EGR2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Immunohistochemistry
Anti-EGR2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Western blot
Antibody (0.03 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry
Anti-EGR2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Immunohistochemistry
Anti-EGR2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Western blot
Antibody (0.03 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry
Anti-EGR2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Immunohistochemistry
Anti-EGR2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3255 concentration 75 ug/ml.
Western blot
Antibody (0.03 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Requested From:
Date Requested: 6/22/2018
Date Requested: 6/22/2018
