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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EGLN3 / PHD3 Antibody (Internal) LS‑C9581
PHD3 antibody LS-C9581 is an unconjugated rabbit polyclonal antibody to PHD3 (EGLN3) from human and rat. Validated for WB.
Antibody
Catalog
Size
Price
LS-C9581-100
100 µl
$590

Popular EGLN3 / PHD3 Products

Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 use at 1:1000 (A) or 1:5000 (B). Detection: Chemiluminescence.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: Western blot
Antibody (0.1 ug/ml) staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HIF Prolyl Hydroxylase 3 Antibody (EG188e/d5) - IHC of HIF Prolyl Hydroxylase 3 in human renal cancer using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Primate
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications: ELISA
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PHD3 antibody LS-C9581 is an unconjugated rabbit polyclonal antibody to PHD3 (EGLN3) from human and rat. Validated for WB.

Specifications

Target
Human EGLN3 / PHD3
Synonyms
EGLN3, HPH-1, HIF prolyl hydroxylase 3, HIF-prolyl hydroxylase 3, HIFPH3, PHD3, EGL nine (C.elegans) homolog 3, Egl nine homolog 3, HIF-PH3, HIFP4H3
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2500)
Immunogen
EGLN3 / PHD3 antibody was raised against a synthetic peptide made to an internal fragment, which represented a portion of human EGL9 homolog 3 encoded within exon 1. [LocusLink ID 112399].
Epitope
Internal
Specificity
Specific for human HIF prolyl hydroxylase 3. Species cross-reactivity: Rat.
Usage
Suitable for use in Western Blot. Western Blot: 1:500-1:2500.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About EGLN3 / PHD3
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Q9H6Z9 NM_022073 NP_071356.1


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Requested From: United States
Date Requested: 10/15/2018

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