Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
EGLN1 / PHD2 antibody was raised against a synthetic peptide made to an N-terminal sequence of the human EGL9 homolog 1 (between residues 1-50). [LocusLink ID 54583].
Specific for human HIF prolyl hydroxylase 2. Species cross-reactivity: Human. This antibody does not appear to work in mouse. Results have been mixed in rat with success in western analysis and immunofluorescence on rat endothelial cells
Suitable for use in Western Blot and Immunohistochemistry.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B.