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Anti-EGFR Antibody (phospho-Tyr1197) LS-C18796


Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular EGFR Antibodies

Anti-EGFR Antibody (clone H11) LS-C26773
Mouse Monoclonal [clone H11] (IgG1)
IHC - Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Immunohistochemistry Image
Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2199
Sheep Polyclonal (IgG) to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry Image
Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2914
Sheep Polyclonal to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human EGFR
Western blot, ELISA


Human EGFR
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:250 - 1:1500)
  • ELISA (1:4000 - 1:20000)

Specificity and Use

EGFR antibody was raised against synthetic peptide
Amino acids 1189-1199 of human EGFR protein.
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kD in size corresponding to phosphorylated EGFR protein by western blotting in the appropriate cell lysate or extract. Less than 5.0% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pY1197 of EGFR protein.


0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About EGFR

P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.

Western blot

Western blot
Anti-EGFR pY1197 Antibody - Western Blot. Western blot using Affinity Purified anti-EGFR pY1197 antibody shows detection of a band at ~170 kD corresponding to human EGFR (arrowhead). Staining is not seen in unstimulated A431 cells (lane 1), but is seen when A431 cells are stimulated with EGF (50 ng/ml for 15 min) (lane 2). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/24/2016

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