Human, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
- Western blot (1 µg/ml)
- ELISA (0.05 µg/ml)
EGFR antibody was raised against synthetic peptide corresponding to aa 1170-1176 (N1170AEpYLRV1176) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173.
Recognizes the N1170AEpYLRV motif corresponding to the major autophosphorylation site of human EGF receptor. Does not cross-react with the non-phosphorylated human, mouse, rat and dog EGF receptor or with unrelated Tyr-phosphorylated proteins. Species cross-reactivity: mouse.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1 ug/ml for AP/BCIP/NBT (MTT) detection; 0.1 ug/ml for HRPO/ECL detection; Use 0.5% Casein, 0.5% BSA in PBST for blocking. Immunoprecipitation: 1-10 ug per 1E6 vanadate treated A431 cells. ELISA: 0.05 ug/ml. Positive control: H2030-03H1-Cell lysate from vanadate-treated HepG2 cells.
PBS, 0.09% sodium azide, PEG, sucrose, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.