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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EGFR Antibody (phospho‑Tyr1173) LS‑C6611
Antibody
EGFR antibody LS-C6611 is an unconjugated mouse monoclonal antibody to EGFR from human, mouse, rat and other species. Validated for ELISA, IP and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C6611-100
100 µg (0.1 mg/ml)
Unavailable

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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Product Description

EGFR antibody LS-C6611 is an unconjugated mouse monoclonal antibody to EGFR from human, mouse, rat and other species. Validated for ELISA, IP and WB. Cited in 1 publication.
About EGFR
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody


Specifications

Target
Human EGFR
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Gel filtration
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (0.05 µg/ml)
Immunogen
EGFR antibody was raised against synthetic peptide corresponding to aa 1170-1176 (N1170AEpYLRV1176) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173.
Epitope
pTyr1173
Specificity
Recognizes the N1170AEpYLRV motif corresponding to the major autophosphorylation site of human EGF receptor. Does not cross-react with the non-phosphorylated human, mouse, rat and dog EGF receptor or with unrelated Tyr-phosphorylated proteins. Species cross-reactivity: mouse.
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1 ug/ml for AP/BCIP/NBT (MTT) detection; 0.1 ug/ml for HRPO/ECL detection; Use 0.5% Casein, 0.5% BSA in PBST for blocking. Immunoprecipitation: 1-10 ug per 1E6 vanadate treated A431 cells. ELISA: 0.05 ug/ml. Positive control: H2030-03H1-Cell lysate from vanadate-treated HepG2 cells.
Presentation
PBS, 0.09% sodium azide, PEG, sucrose, 40% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Dasatinib and Doxorubicin Treatment of Sarcoma Initiating Cells: A Possible New Treatment Strategy. Aggerholm-Pedersen N, Demuth C, Safwat A, Meldgaard P, Kassem M, Sandahl Sorensen B. Stem cells international. 2016 2016:9601493. (WB; Human) [Full Text Article] [PubMed:26788073] [PMC:PMC4693025]

Customer Reviews (0)


Requested From: 
Date Requested: 6/24/2018

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