Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
IHC - Paraffin
Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
EGFR antibody was raised against synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor (KLH).
Recognizes endogenous levels of human EGF receptor at ~175kD only when phosphorylated at Tyr1068 at 175kD. The antibody may cross-react with other activated EGF receptor family members (e. g., ErbB2), and cross-reacts slightly with activated PDGF receptor.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50-350.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.
Temporal regulation of EGF signalling networks by the scaffold protein Shc1. Zheng Y, Zhang C, Croucher DR, Soliman MA, St-Denis N, Pasculescu A, Taylor L, Tate SA, Hardy WR, Colwill K, Dai AY, Bagshaw R, Dennis JW, Gingras AC, Daly RJ, Pawson T. Nature. 2013 Jul;499:166-71. [Full Text Article]