LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
EGFR Antibody LS‑C6603
EGFR antibody LS-C6603 is an unconjugated mouse monoclonal antibody to human EGFR. Validated for ICC, IHC, IP, Neut and WB.
Catalog
Size
Price
LS-C6603-250
250 µg (1 mg/ml)
$605
Description
EGFR antibody LS-C6603 is an unconjugated mouse monoclonal antibody to human EGFR. Validated for ICC, IHC, IP, Neut and WB.
Target
Human EGFR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (10 µg/ml)
  • ICC (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • Neutralization
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EGFR antibody was raised against human A431 membrane proteins.
Specificity
Recognizes the external domain of EGF receptor on all human cells. Competes with EGF and TGF-a for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors. Species cross-reactivity: does not react with EGF receptors on mouse or rat cells.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Neutralization. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates the EGF receptor from 500 ug of non-stimulated A431 RIPA lysate. Immunocytochemistry: 10 ug/ml shows positive immunostaining for the EGF receptor in A431 cells fixed with 4% para-formaldehyde.
Presentation
Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride
Reconstitution
250 µl Sterile 40-50% glycerol, ddH2O
Storage
Lyophilized and resuspended forms: store at -20°C.
Restrictions
For research use only.
About EGFR
P00533 NM_005228 NP_005219.2

Popular EGFR Products

Anti-EGFR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Anti-EGFR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Antibody
Species: Human, Monkey
Applications: Immunoprecipitation, Blocking, Inhibition
Formalin-fixed, paraffin-embedded human squamous cell carcinoma of lung stained with peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Anti-EGFR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 12/16/2018
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy