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Anti-EGFR Antibody LS-C6603

Ordering

Wt. Vol. Conc. Price
250 µg 250 µl 1 mg/ml $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG1) to Human EGFR
Human
IHC, ICC, Western blot, Immunoprecipitation, Neutralization
Unconjugated

Details

Human EGFR
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Protein G purified

Applications

IHC (10 µg/ml), ICC (10 µg/ml), Western blot, Immunoprecipitation, Neutralization

Specificity and Use

EGFR antibody was raised against human A431 membrane proteins.
Recognizes the external domain of EGF receptor on all human cells. Competes with EGF and TGF-a for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors. Species cross-reactivity: does not react with EGF receptors on mouse or rat cells.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Neutralization. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates the EGF receptor from 500 ug of non-stimulated A431 RIPA lysate. Immunocytochemistry: 10 ug/ml shows positive immunostaining for the EGF receptor in A431 cells fixed with 4% para-formaldehyde.

Packaging

Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride
250 µl Sterile 40-50% glycerol, ddH2O
Lyophilized and resuspended forms: -20°C
For research use only.

About EGFR

P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.

Requested From: United States
Date Requested: 9/28/2016

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