(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC (10 µg/ml)
- ICC (10 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
EGFR antibody was raised against human A431 membrane proteins.
Recognizes the external domain of EGF receptor on all human cells. Competes with EGF and TGF-a for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors. Species cross-reactivity: does not react with EGF receptors on mouse or rat cells.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Neutralization. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitates the EGF receptor from 500 ug of non-stimulated A431 RIPA lysate. Immunocytochemistry: 10 ug/ml shows positive immunostaining for the EGF receptor in A431 cells fixed with 4% para-formaldehyde.
Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride
250 µl Sterile 40-50% glycerol, ddH2O
Lyophilized and resuspended forms: store at -20°C.
For research use only.