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Catalog Number Size Price
LS-C413525-100 100 µl (1 mg/ml) $321 
EGFR Antibody - Immunofluorescent analysis of EGFR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EGFR Antibody - Western blot analysis of EGFR expression in A431 (A); HeLa (B) whole cell lysates.
EGFR Antibody - Immunoprecipitation of EGFR from 0.5mg HeLa whole cell extract lysate; using Anti-EGFR Antibody.
EGFR Antibody - Immunofluorescent analysis of EGFR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EGFR Antibody - Western blot analysis of EGFR expression in A431 (A); HeLa (B) whole cell lysates.
EGFR Antibody - Immunoprecipitation of EGFR from 0.5mg HeLa whole cell extract lysate; using Anti-EGFR Antibody.
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Monoclonal Mouse anti‑Human EGFR Antibody (IF, WB) LS‑C413525

Monoclonal Mouse anti‑Human EGFR Antibody (IF, WB) LS‑C413525

Antibody:
EGFR Mouse anti-Human Monoclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413525-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EGFR Mouse anti-Human Monoclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EGFR antibody LS-C413525 is an unconjugated mouse monoclonal antibody to human EGFR. Validated for ICC, IF, IP and WB.
Target
Human EGFR
Synonyms
EGFR | EGF Receptor | ERBB | ERBB1 | MENA | PIG61 | Proto-oncogene c-ErbB-1 | V-erb-b homolog | EGF-R | HER1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human EGFR.
Specificity
Recognizes endogenous levels of EGFR protein.
Applications
  • ICC (1:100 - 1:200)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:1000 - 1:3000)
  • Immunoprecipitation (1:100 - 1:200)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGFR
P00533 NM_005228 NP_005219.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024