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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EGFR Antibody (clone 199.12, Azide‑free) LS‑C88157
Antibody
EGFR antibody LS-C88157 is an unconjugated mouse monoclonal antibody to human EGFR. Validated for IP and WB.
Catalog
Size
Price
LS-C88157-100
100 µl
Unavailable
LS-C88157-200
200 µl
Unavailable

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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Product Description

EGFR antibody LS-C88157 is an unconjugated mouse monoclonal antibody to human EGFR. Validated for IP and WB.
About EGFR
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody


Specifications

Target
Human EGFR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [199.12]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • Western blot (2 - 4 µg/ml)
  • Immunoprecipitation (2 - 4 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
EGFR antibody was raised against extracellular domain of human recombinant EGFR protein.
Specificity
Extracellular domain
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
10mM PBS, pH 7.4
Storage
Stable for 36 months when stored at or below 0°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/21/2018

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