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Anti-EGFR Antibody (aa1166-1180) IHC-plus™ LS-B648

Note: This antibody replaces LS-C18794

Ordering

Wt. Vol. Conc. Price
- 50 µl 85 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EGFR Antibodies

Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2199
Sheep Polyclonal (IgG) to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EGFR Antibody (clone H11) LS-C26773
Mouse Monoclonal [clone H11] (IgG1)
Human
IHC - Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2914
Sheep Polyclonal to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human EGFR
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human EGFR
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Sterile filtered
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:50000)

Specificity and Use

aa1166-1180
Peptide synthesized using conventional technology. The sequence of the epitope is: NH2-C-S-L-D-N-P-D-Y-Q-Q-D-F-F-P-K-E-COOH and corresponds to amino acids 1166-1180 of the EGFR molecule. This maps to a region near the carboxy-terminus which is identical in human, mouse and rat EGFR. The amino terminal cysteine was synthesized to facilitate carrier coupling.
Immunohistochemistry: LS-B648 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B648 was determined to be 1:500.

Packaging

Serum.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About EGFR

P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.

Immunohistochemistry

Immunohistochemistry
Anti-EGFR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B648 dilution 1:500.

Western blot

Western blot
Western Blot - Anti-EGFR Antibody. Western blot of anti-EGFR antibody shows detection of a band at ~170 kD corresponding to human EGFR present in unstimulated (lane 1) and EGF (50 ng/ml for 15 min) stimulated (lane 2) A431 whole cell lysates (arrowhead). Approximately 30 ug of lysate was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).

Western blot

Western blot
Immunoprecipitation/Western Blot - Anti-EGFR Antibody. Combined immunoprecipitation and Western blot of anti-EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15 at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 1/24/2017

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