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Anti-EGF Antibody (Biotin) LS-C104401

Note: This antibody replaces LS-C36261, LS-C42270, LS-C54004, LS-C23142, LS-C23144, LS-C23155
Catalog Size Price
LS-C104401-25 25 µg Unavailable
LS-C104401-50 50 µg Unavailable

Related Products

154 EGF Antibodies

Most Popular EGF Antibodies

Anti-EGF Antibody LS-C104654
Rabbit Polyclonal to Human EGF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-EGF Antibody LS-C104712
Rabbit Polyclonal to Rat EGF
Rat
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-EGF Antibody (clone 9D7F11) IHC-plus™ LS-B4124
Mouse Monoclonal [clone 9D7F11] (IgG1) to Human EGF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EGF Antibody (aa690-720, FITC) LS-C213687
Rabbit Polyclonal (IgG) to Human EGF
Human, Mouse
IHC, Western blot, ELISA
FITC Conjugated
Anti-EGF Antibody (aa106-119) IHC-plus™ LS-B11905
Goat Polyclonal to Human EGF
Human, Monkey, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Goat Polyclonal to Mouse EGF
Mouse
Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Biotin Conjugated

Details

Mouse EGF
Goat
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)

Specificity and Use

EGF antibody was raised against e.coli derived recombinant Murine EGF.
Mouse EGF
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF. To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine EGF (LS-C104392) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF. Western Blot: To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About EGF

P01133 NM_001963 NP_001954.2

EGF Antibody, Beta-urogastrone Antibody, HOMG4 Antibody, URG Antibody, Epidermal growth factor Antibody, Pro-epidermal growth factor Antibody

EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor.

Western blot

Western blot
Western Blot (reducing) of EGF antibody LS-C104392. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of EGF antibody LS-C104392. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of EGF antibody LS-C104392. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/19/2017

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