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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EFNB3 / Ephrin B3 Antibody (Internal) LS‑C6553 Best Seller
Ephrin B3 antibody LS-C6553 is an unconjugated rabbit polyclonal antibody to Ephrin B3 (EFNB3) from human and mouse. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C6553-100
100 µg (0.25 mg/ml)
$605
Description
Ephrin B3 antibody LS-C6553 is an unconjugated rabbit polyclonal antibody to Ephrin B3 (EFNB3) from human and mouse. Validated for IHC, IP and WB.
Target
Mouse EFNB3 / Ephrin B3
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1 - 5 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from an internal sequence of the mouse ephrin-B3 protein.
Epitope
Internal
Specificity
Recognizes mouse ephrin-B3 protein. Species cross-reactivity: human. Reactivity is confirmed with a chimeric protein consisting of the extracellular domain of human ephrin-B3 and the Fc region of human IgG1. Cross-reactivity with human ephrin-B3 is confirmed with IHC experiments on human tissue sections and this reactivity was expected because of the 98% shared amino acid identity in the extracellular domains. and.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-5 ug/ml. Immunoprecipitation: 5-10 ug/ IP reaction. Immunohistochemistry: 5-10 ug/m. Confirmed with human tissue sections.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About EFNB3 / Ephrin B3
Q15768 NM_001406 NP_001397.1

Popular EFNB3 / Ephrin B3 Products

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EFNB3 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Mouse, Human
Applications: IHC, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human liver tissue at dilution 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunofluorescent analysis of Ephrin B3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Applications: ICC, Immunofluorescence, Western blot

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Requested From: United States
Date Requested: 12/15/2018
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