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Anti-EEF2K Antibody (aa1-268) LS-C186215

Catalog Size Price
LS-C186215-100 100 µl (1 mg/ml) Unavailable

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40 EEF2K Antibodies

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Anti-EEF2K Antibody (aa336-385) IHC-plus™ LS-B9878
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Anti-EEF2K Antibody (aa1-50) LS-C288474
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Anti-EEF2K Antibody LS-C334041
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Anti-EEF2K Antibody LS-C412495
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human EEF2K
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human EEF2K
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)

Specificity and Use

EEF2K antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 268 of EEF2K (SwissProt O00418). Percent identity by BLAST analysis: Human (100%); Bovine (90%).
aa1-268
Human EEF2K
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About EEF2K

O00418 NM_013302 NP_037434.1

EEF2K Antibody, EEF-2K Antibody, Elongation factor-2 kinase Antibody, EEF-2 kinase Antibody, HSU93850 Antibody

EEF2K, also known as Ca2+/CaM-dependent protein kinase III, is a Alpha-kinase. EEF2K, a member of the calmodulin-mediated signaling pathway, catalyzes the movement of the ribosome along mRNA during translation in eukaryotic cells. At least three mRNA variants have been reported -- a major 3.1-kb transcript and minor 6.1- and 2.5-kb transcripts. EEF2K activity has been shown to be increased in several types of cancer, including breast cancer.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded BT483 xenograft using EEF2K antibody at 1:500 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed A431 using EEF2K antibody at 1:200 dilution.

Western blot

Western blot
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. EEF2K antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: A431 , B: H1299, C: Hela, D: Hep G2 . 7.5% SDS PAGE. EEF2K antibody diluted at 1:5000.

Requested From: 
Date Requested: 6/24/2017

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