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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EEF1D Antibody (clone 4B12) LS‑C197110
EEF1D antibody LS-C197110 is an unconjugated mouse monoclonal antibody to human EEF1D. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C197110-100
100 µg
Unavailable

Popular EEF1D Products

Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
IHC of paraffin-embedded AdCA, using EEF1D antibody at 1:250 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded mouse brain tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

EEF1D antibody LS-C197110 is an unconjugated mouse monoclonal antibody to human EEF1D. Validated for ELISA, IF and WB.
About EEF1D
EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. P29692 NM_032378 NP_115754.3

EEF1D Antibody, Antigen NY-CO-4 Antibody, EF-1D Antibody, EF-1-delta Antibody, Elongation factor 1-delta Antibody, FP1047 Antibody, EF1D Antibody


Specifications

Target
Human EEF1D
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [4B12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Immunogen
EEF1D antibody was raised against eEF1D (NP_115754, aa1-91 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human EEF1D
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EEF1D is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/22/2018

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