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Antibodies > ECI1 / DCI > LS-C312910
 
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Catalog Number Size Price
LS-C312910-10 10 µg $318 
LS-C312910-100 100 µg (0.5 mg/ml) $470 
ECI1 / DCI Antibody - DCI / ECI1 antibody. IHC(P): Human Breast Cancer Tissue.
ECI1 / DCI Antibody - DCI / ECI1 antibody. ICC: A549 Cell.
ECI1 / DCI Antibody - DCI / ECI1 antibody. ICC: HELA Cell.
ECI1 / DCI Antibody - IF analysis of DCI using anti-DCI antibody DCI was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-DCI Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ECI1 / DCI Antibody - IF analysis of DCI using anti-DCI antibody DCI was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-DCI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ECI1 / DCI Antibody - Western blot analysis of DCI using anti-DCI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: A549 Whole Cell Lysate, Lane 4: SMMC Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: HT1080 Whole Cell Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.
ECI1 / DCI Antibody - WB of DCI / ECI1 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Human Placenta Tissue Lysate. Lane 3: A549 Cell Lysate. Lane 4: SMMC Cell Lysate . Lane 5: COLO320 Cell Lysate . Lane 6: HELA Cell Lysate . Lane 7: HT1080 Cell Lysate.
ECI1 / DCI Antibody - DCI / ECI1 antibody. IHC(P): Human Breast Cancer Tissue.
ECI1 / DCI Antibody - DCI / ECI1 antibody. ICC: A549 Cell.
ECI1 / DCI Antibody - DCI / ECI1 antibody. ICC: HELA Cell.
ECI1 / DCI Antibody - IF analysis of DCI using anti-DCI antibody DCI was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-DCI Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ECI1 / DCI Antibody - IF analysis of DCI using anti-DCI antibody DCI was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-DCI Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ECI1 / DCI Antibody - Western blot analysis of DCI using anti-DCI antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: A549 Whole Cell Lysate, Lane 4: SMMC Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: HT1080 Whole Cell Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.
ECI1 / DCI Antibody - WB of DCI / ECI1 antibody. Lane 1: Rat Liver Tissue Lysate. Lane 2: Human Placenta Tissue Lysate. Lane 3: A549 Cell Lysate. Lane 4: SMMC Cell Lysate . Lane 5: COLO320 Cell Lysate . Lane 6: HELA Cell Lysate . Lane 7: HT1080 Cell Lysate.
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Polyclonal Rabbit anti‑Human ECI1 / DCI Antibody (aa272‑290, IHC, WB) LS‑C312910

Polyclonal Rabbit anti‑Human ECI1 / DCI Antibody (aa272‑290, IHC, WB) LS‑C312910

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Note: This antibody replaces LS-C388338
Antibody:
ECI1 / DCI Rabbit anti-Human Polyclonal (aa272-290) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312910-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ECI1 / DCI Rabbit anti-Human Polyclonal (aa272-290) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
DCI antibody LS-C312910 is an unconjugated rabbit polyclonal antibody to DCI (ECI1) (aa272-290) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.
Target
Human ECI1 / DCI
Synonyms
ECI1 | 3,2 trans-enoyl-CoA isomerase | 3,2-trans-enoyl-CoA isomerase | Acetylene-allene isomerase | DCI | Dodecenoyl-CoA isomerase | Enoyl-CoA delta isomerase 1 | D3,D2-enoyl-CoA isomerase
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human DCI(272-290 aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.
Epitope
aa272-290
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ECI1 / DCI
NM_001919 NP_001910.2

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ECI1 / DCI Antibody (N‑Terminus) LS‑C81259
Species: Human
Applications: Western blot
ECI1 / DCI Antibody - Immunofluorescence analysis of A549 cells.
ECI1 / DCI Antibody LS‑C331354
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Applications: IHC, Immunofluorescence, Western blot
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
SFTPC / Surfactant Protein C ELISA Kit
Human SFTPC / Surfactant Protein C (Sandwich ELISA) ELISA Kit - LS-F12438
Reactivity: Human
Range: 0.469-30 ng/ml
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See all ECI1 / DCI Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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