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ECH1 Antibody (aa51‑100) LS‑C80585
ECH1 antibody LS-C80585 is an unconjugated rabbit polyclonal antibody to ECH1 from human, mouse, rat and other species. Validated for WB.
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Species:
Human, Rat
Applications:
ICC, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Product Description
ECH1 antibody LS-C80585 is an unconjugated rabbit polyclonal antibody to ECH1 from human, mouse, rat and other species. Validated for WB.
About ECH1
ECH1 is a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome.
Q13011
NM_001398
NP_001389.2
ECH1 Antibody, Dienoyl-CoA isomerase Antibody, HPXEL Antibody
Specifications
Target
Human ECH1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Dog (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Protein A purified
Modifications
Unmodified
Applications
- Western blot
- (applications tested for the base form of this product only)
Immunogen
ECH1 antibody was raised against synthetic peptide located between aa51-100 of human ECH1 (Q13011, NP_001389). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Marmoset, Mouse, Rat, Panda, Bovine, Pig, Guinea pig (100%); Dog (92%); Opossum (90%); Rabbit (85%); Bat (84%).
Blocking Peptide
LS-E16025 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80585. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C80585. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa51-100
Specificity
Human ECH1
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 2.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
