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ECEL1 antibody LS-C9261 is an unconjugated rabbit polyclonal antibody to rat ECEL1. Validated for ELISA and WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, ELISA
Product Description
ECEL1 antibody LS-C9261 is an unconjugated rabbit polyclonal antibody to rat ECEL1. Validated for ELISA and WB.
About ECEL1
ECEL1 is a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms.
O95672
NM_004826
NP_004817.2
ECEL1 Antibody, XCE Antibody, Xce protein Antibody, DINE Antibody, ECEX Antibody, X converting enzyme Antibody
Specifications
Target
Rat ECEL1
Host
Rabbit
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (1:10000 - 1:100000)
Immunogen
ECEL1 antibody was raised against a 17-aa peptide sequence, mapping near the cytoplasmic N-terminus of rat DINE was synthesized, coupled to KLH.
Epitope
N-Terminus
Specificity
Recognizes rat DINE. Species sequence homology: Mouse (94%) and human (88%). No significant sequence homology is seen with NEP, NEPLs or ECEs or other proteins.
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.
Presentation
Tris, pH 7.5, 0.1% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
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