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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EBP Antibody (aa77‑126) LS‑C80927
EBP antibody LS-C80927 is an unconjugated rabbit polyclonal antibody to EBP from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80927-50
50 µg
$365

Popular EBP Products

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EBP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

EBP antibody LS-C80927 is an unconjugated rabbit polyclonal antibody to EBP from human, mouse, rat and other species. Validated for WB.
About EBP
EBP is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. Q15125 NM_006579 NP_006570.1

EBP Antibody, CDPX2 Antibody, CPXD Antibody, CHO2 Antibody, Cholestenol Delta-isomerase Antibody, CPX Antibody, D8-D7 sterol isomerase Antibody, Emopamil-binding protein Antibody, Sterol 8-isomerase Antibody


Specifications

Target
Human EBP
Host
Rabbit
Reactivity
Human, Mouse, Rat, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
EBP antibody was raised against synthetic peptide located between aa77-126 of human EBP (Q15125, NP_006570). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Galago, Elephant (100%); Monkey, Marmoset, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig (92%); Guinea pig (85%); Mouse, Rat, Hamster (84%).
Blocking Peptide
LS-E17002 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80927. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa77-126
Specificity
Human EBP
Usage
ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/22/2018

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