Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone DNT27] (IgG2a,k) to Mouse EBI3 / IL-27B
IHC - Paraffin, Western blot
Mouse EBI3 / IL-27B
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [DNT27]
IHC - Paraffin (10 µg/ml)
Specificity and Use
EBI3 / IL-27B antibody was raised against mouse EBI3
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Aqueous buffer, 0.09% sodium azide. Sourced from tissue culture supernatant.
IL-35 mitigates murine acute graft-versus-host disease with retention of graft-versus-leukemia effects. Liu Y, Wu Y, Wang Y, Cai Y, Hu B, Bao G, Fang H, Zhao L, Ma S, Cheng Q, Song Y, Liu Y, Zhu Z, Chang H, Yu X, Sun A, Zhang Y, Vignali DA, Wu D, Liu H. Leukemia. 2015 29:939-46. (IHC; Mouse)[Full Text Article]
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells.
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5764 concentration 10 ug/ml.
Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.