Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone DNT27] (IgG2a,k) to Mouse EBI3 / IL-27B
IHC - Paraffin, Western blot
Mouse EBI3 / IL-27B
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [DNT27]
IHC - Paraffin (10 µg/ml)
Specificity and Use
EBI3 / IL-27B antibody was raised against mouse EBI3
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Aqueous buffer, 0.09% sodium azide. Sourced from tissue culture supernatant.
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells.
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5764 concentration 10 ug/ml.
Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.