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Anti-EBI3 / IL-27B Antibody (clone DNT27) IHC-plus™ LS-B5764

Note: This antibody replaces LS-C107123
Catalog Size Price
LS-B5764-50 50 µg (0.5 mg/ml) Unavailable

Related Products

80 EBI3 / IL-27B Antibodies

Most Popular EBI3 / IL-27B Antibodies

Anti-EBI3 / IL-27B Antibody (C-Terminus) LS-C82902
Rabbit Polyclonal (IgG) to Human EBI3 / IL-27B
Human, Mouse
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry of EBI3 in human spleen tissue with LS-C82902 at 2.5 µg/ml.
Anti-EBI3 / IL-27B Antibody (Internal) LS-C82906
Rabbit Polyclonal (IgG) to Human EBI3 / IL-27B
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry of EBI3 in human spleen tissue with LS-C82906 at 2.5 µg/ml.
Anti-EBI3 / IL-27B Antibody (clone DNT27) IHC-plus™ LS-B5764
Rat Monoclonal [clone DNT27] (IgG2a,k) to Mouse EBI3 / IL-27B
Mouse, Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5764 concentration 10 ug/ml.
Anti-EBI3 / IL-27B Antibody (aa200-230) LS-C148525
Rabbit Polyclonal to Mouse EBI3 / IL-27B
Mouse, Rat
IHC, IHC - Paraffin, Western blot
Unconjugated
Western blot of EBI3 in RAW cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148525 at 0.5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Anti-EBI3 / IL-27B Antibody (aa120-170) IHC-plus™ LS-B7207
Rabbit Polyclonal (IgG) to Human EBI3 / IL-27B
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-EBI3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7207 dilution 1:100.

100% Guaranteed Publications (1)
Rat Monoclonal [clone DNT27] (IgG2a,k) to Mouse EBI3 / IL-27B
Mouse, Human
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Mouse EBI3 / IL-27B
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [DNT27]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

EBI3 / IL-27B antibody was raised against mouse EBI3
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

Aqueous buffer, 0.09% sodium azide. Sourced from tissue culture supernatant.
Store at 4°C, avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
IL-35 mitigates murine acute graft-versus-host disease with retention of graft-versus-leukemia effects. Liu Y, Wu Y, Wang Y, Cai Y, Hu B, Bao G, Fang H, Zhao L, Ma S, Cheng Q, Song Y, Liu Y, Zhu Z, Chang H, Yu X, Sun A, Zhang Y, Vignali DA, Wu D, Liu H. Leukemia. 2015 29:939-46. (IHC; Mouse) [Full Text Article] [PubMed:25363669] [PMC:PMC4391991]

About EBI3 / IL-27B

Q14213 NM_005755 NP_005746.2

EBI3 Antibody, Epstein-Barr virus induced 3 Antibody, IL-27 subunit beta Antibody, IL27 subunit Antibody, IL27B Antibody, Interleukin-27 subunit beta Antibody, IL-27B Antibody, Cytokine receptor Antibody, EBV-induced gene 3 protein Antibody, IL35 subunit Antibody

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells.

Immunohistochemistry

Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5764 concentration 10 ug/ml.
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5764 concentration 10 ug/ml.

Western blot

Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.
Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.

Requested From: 
Date Requested: 5/27/2018

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