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Anti-EBAG9 / RCAS1 Antibody (aa240-251) IHC-plus™ LS-B2762

Note: This antibody replaces LS-C54496, LS-C65147

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EBAG9 / RCAS1 Antibodies

Anti-EBAG9 / RCAS1 Antibody (C-Terminus) LS-C50471
Rabbit Polyclonal to Human EBAG9 / RCAS1
Human
IHC
Unconjugated
Immunohistochemistry Image
Anti-EBAG9 / RCAS1 Antibody (aa240-251) IHC-plus™ LS-B2762
Goat Polyclonal to Human EBAG9 / RCAS1
Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EBAG9 / RCAS1 Antibody (aa88-117, PE) LS-C259228
Rabbit Polyclonal (IgG) to Human EBAG9 / RCAS1
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human EBAG9 / RCAS1
Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human EBAG9 / RCAS1
Goat
Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Rat, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (1:64000)

Specificity and Use

EBAG9 / RCAS1 antibody was raised against synthetic peptide C-KEAQRLMKKEQN from the C-terminus of human EBAG9 (NP_004206.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%); Rat, Lizard, Xenopus (92%); Pufferfish, Ajellomyces (83%).
LS-E26119 - Lyophilized - 100 µg - $145.00
aa240-251
Human EBAG9.
Immunohistochemistry: LS-B2762 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2762 was determined to be 3.75 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About EBAG9 / RCAS1

O00559 NM_004215 NP_004206.1

EBAG9 Antibody, EB9 Antibody, PDAF Antibody, RCAS1 Antibody

EBAG9 / RCAS1 was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.

Immunohistochemistry

Immunohistochemistry
Anti-EBAG9 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2762 concentration 75 ug/ml.

Western blot

Western blot
Antibody (0.2 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/3/2016

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