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Anti-E6AP / UBE3A Antibody (aa180-229) LS-C110728

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

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43 E6AP / UBE3A Antibodies

Most Popular E6AP / UBE3A Antibodies

Anti-E6AP / UBE3A Antibody (aa1-851) LS-C19980
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Anti-E6AP / UBE3A Antibody (aa836-865) LS-C99579
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Anti-E6AP / UBE3A Antibody (clone 2F6) LS-C133508
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human E6AP / UBE3A
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Anti-E6AP / UBE3A Antibody (N-Terminus) IHC-plus™ LS-B13693
Rabbit Polyclonal to Human E6AP / UBE3A
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Anti-E6AP / UBE3A Antibody (aa342-585) IHC-plus™ LS-B14302
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Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human E6AP / UBE3A
Human, Monkey, Rat, Bat, Zebrafish, Drosophila
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human E6AP / UBE3A
Rabbit
Human, Monkey, Rat, Bat, Zebrafish, Drosophila (tested or 100% immunogen sequence identity)
Mouse, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

E6AP / UBE3A antibody was raised against synthetic peptide located between aa180-229 of human UBE3A (Q05086, NP_000453). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Zebrafish, Drosophila (100%); Galago, Mouse, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (92%).
LS-E15805 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110728. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa180-229
Human UBE3A
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About E6AP / UBE3A

Q05086 NM_000462 NP_000453.2

UBE3A Antibody, ANCR Antibody, E6-ap isoform-III Antibody, E6AP ubiquitin-protein ligase Antibody, EPVE6AP Antibody, HPVE6A Antibody, AS Antibody, CTCL tumor antigen se37-2 Antibody, E6-AP Antibody, E6AP Antibody, Ubiquitin protein ligase E3A Antibody, Ubiquitin-protein ligase E3A Antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.

Western blot

Western blot
UBE3A antibody LS-C110728 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/24/2017

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