LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
E2F5 Antibody (aa134‑183) LS‑C30420
E2F5 antibody LS-C30420 is an unconjugated rabbit polyclonal antibody to E2F5 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C30420-50
50 µg (1 mg/ml)
Unavailable

Popular E2F5 Products

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-E2F5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11416 dilution 1:50.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-E2F5 mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

E2F5 antibody LS-C30420 is an unconjugated rabbit polyclonal antibody to E2F5 from human, mouse, rat and other species. Validated for WB.
About E2F5
E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. Q15329 NM_001951 NP_001942.2

E2F5 Antibody, Transcription factor E2F5 Antibody, E2F-5 Antibody


Specifications

Target
Human E2F5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
E2F5 antibody was raised against synthetic peptide located between aa134-183 of human E2F5 (Q15329, NP_001942). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Bat, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (92%); Zebrafish (84%).
Blocking Peptide
LS-E10917 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C30420. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa134-183
Specificity
Human E2F5
Presentation
Lyophilized.
Reconstitution
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number