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Anti-E2F3 Antibody (clone 5F7) LS-C197095

Catalog Size Price
LS-C197095-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 5F7] (IgG1,k) to Human E2F3
Human
Western blot, ELISA
Unconjugated

Details

Human E2F3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [5F7]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

E2F3 antibody was raised against e2F3 (NP_001940, aa336-425 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human E2F3
Sandwich ELISA: Recombinant protein, Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About E2F3

O00716 NM_001949 NP_001940.1

E2F3 Antibody, E2F transcription factor 3 Antibody, KIAA0075 Antibody, Transcription factor E2F3 Antibody, E2F-3 Antibody

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

Western blot

Western blot
E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 6/23/2017

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