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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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E2F3 Antibody (clone 5F7) LS‑C197095
E2F3 antibody LS-C197095 is an unconjugated mouse monoclonal antibody to human E2F3. Validated for ELISA and WB.
Catalog
Size
Price
LS-C197095-100
100 µg
Unavailable

Popular E2F3 Products

Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Gel shift
Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 µg/ml.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot

Product Description

E2F3 antibody LS-C197095 is an unconjugated mouse monoclonal antibody to human E2F3. Validated for ELISA and WB.
About E2F3
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. O00716 NM_001949 NP_001940.1

E2F3 Antibody, E2F transcription factor 3 Antibody, KIAA0075 Antibody, Transcription factor E2F3 Antibody, E2F-3 Antibody


Specifications

Target
Human E2F3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [5F7]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
E2F3 antibody was raised against e2F3 (NP_001940, aa336-425 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human E2F3
Usage
Sandwich ELISA: Recombinant protein, Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.
E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.

Western blot

E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.
E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.

Western blot

E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.
E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.

Western blot

E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.
E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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