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Anti-E2F3 Antibody (C-Terminus) IHC-plus™ LS-B1522

Note: This antibody replaces LS-C30196

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular E2F3 Antibodies

Anti-E2F3 Antibody (C-Terminus) IHC-plus™ LS-B1522
Rabbit Polyclonal (IgG) to Human E2F3
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-E2F3 Antibody (Internal) LS-C336201
Rabbit Polyclonal to Human E2F3
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-E2F3 Antibody (C-Terminus, Biotin) LS-C436422
Rabbit Polyclonal (IgG) to Human E2F3
Human, Chimpanzee, Gibbon, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC, IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human E2F3
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human E2F3
Rabbit
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Monkey, Rabbit, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

E2F3 antibody was raised against synthetic peptide from C-Terminus of human E2F3 (Q68DT0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig (100%); Marmoset, Rabbit, Turkey, Zebra finch, Chicken, Platypus, Lizard (92%); Panda (90%); Xenopus (84%).
LS-E13665 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1522. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human E2F3
Immunohistochemistry: LS-B1522 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1522 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About E2F3

O00716 NM_001949 NP_001940.1

E2F3 Antibody, E2F transcription factor 3 Antibody, KIAA0075 Antibody, Transcription factor E2F3 Antibody, E2F-3 Antibody

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

Immunohistochemistry

Immunohistochemistry
Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western analysis of fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/26/2017

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