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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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E2F3 Antibody (C‑Terminus) IHC‑plus™ LS‑B1522 Best Seller
Note: This antibody replaces LS-C30196
E2F3 antibody LS-B1522 is an unconjugated rabbit polyclonal antibody to E2F3 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1522-50
50 µg (1 mg/ml)
Unavailable

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Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot

Product Description

E2F3 antibody LS-B1522 is an unconjugated rabbit polyclonal antibody to E2F3 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About E2F3
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. O00716 NM_001949 NP_001940.1

E2F3 Antibody, E2F transcription factor 3 Antibody, KIAA0075 Antibody, Transcription factor E2F3 Antibody, E2F-3 Antibody


Specifications

Target
Human E2F3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rabbit, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E2F3 antibody was raised against synthetic peptide from C-Terminus of human E2F3 (Q68DT0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig (100%); Marmoset, Rabbit, Turkey, Zebra finch, Chicken, Platypus, Lizard (92%); Panda (90%); Xenopus (84%).
Blocking Peptide
LS-E13665 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1522. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human E2F3
Usage
Immunohistochemistry: LS-B1522 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1522 was determined to be 5 ug/ml.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E2F3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1522 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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