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Anti-E2F1 Antibody (phospho-Ser364) IHC-plus™ LS-B52

Note: This antibody replaces LS-C18900, LS-C65131, LS-C56901

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular E2F1 Antibodies

Anti-E2F1 Antibody (clone KH20, KH95) LS-C14959
Mouse Monoclonal [clone KH20, KH95] (IgG) to Human E2F1
Human, Xenopus
Western blot, Immunoprecipitation
Unconjugated
Anti-E2F1 Antibody (Internal) IHC-plus™ LS-B6744
Rabbit Polyclonal to Human E2F1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-E2F1 Antibody (clone 8G9) LS-C169098
Mouse Monoclonal [clone 8G9] (IgG1) to Human E2F1
Human, Mouse
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human E2F1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human E2F1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:250 - 1:2000)
  • ELISA (1:20000 - 1:100000)

Specificity and Use

E2F1 antibody was raised against synthetic peptide from human E2F1.
pSer364
Amino acids 360-369 of Human E2F-1.
Immunohistochemistry: LS-B52 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B52 was determined to be 10 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About E2F1

Q01094 NM_005225 NP_005216.1

E2F1 Antibody, E2F transcription factor 1 Antibody, PRB-binding protein E2F-1 Antibody, RBAP-1 Antibody, RBBP3 Antibody, RBBP-3 Antibody, Transcription factor E2F1 Antibody, RBAP1 Antibody, E2F-1 Antibody, PBR3 Antibody

E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.

Immunohistochemistry

Immunohistochemistry
Anti-E2F1 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B52 concentration 10 ug/ml.

Western blot

Western blot
Western blot of Affinity Purified anti-E2F-1 pS364 antibody shows detection of a band at ~47 kD corresponding to phosphorylated E2F-1 in induced cell lysates. Panel A shows reactivity using a control antibody reactive to all forms of E2F (arrowheads). Panel B shows specific reactivity against phosphorylated E2F-1 (arrowheads) using our anti-E2F-1 pS364 antibody. Lysates are as follows: CRE/E2F-1 are CRE cells derived from mouse NIH3T3 line transfected with human E2F-1, NIH-3T3 used as a negative control, and MDA-MB-231 cells are a human breast cancer line. As indicated each lysate was prepared from untreated cells and cells treated with 2 uM Doxorubicin used as a DNA damaging agent. In addition the MDA-MB-231 cells were also treated with genistein, a mild DNA damaging agent. The figure shows the same membrane first probed with the anti-E2F-1 pS364 antibody used at a 1:250 dilution, then stripped and re-probed with the pan E2F antibody used as a positive control. The positive control antibody clearly shows an E2F-1 band in all human cell lines, but not mouse cells. Treatment with doxorubicin increases expression of E2F-1 as shown in Panel A. Images were overlapped to confirm that anti-E2F-1 pS364 staining shown treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 LS-C60865 for 45 min at room temperature for ECL detection. Personal Communication, XiaoHe Yang, Univ. Oklahoma.

Requested From: United States
Date Requested: 12/9/2016

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