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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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E2F1 Antibody LS‑C14968
Antibody
E2F1 antibody LS-C14968 is an unconjugated mouse monoclonal antibody to human E2F1. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C14968-100
100 µg
Unavailable

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Product Description

E2F1 antibody LS-C14968 is an unconjugated mouse monoclonal antibody to human E2F1. Validated for IHC, IP and WB.
About E2F1
E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Q01094 NM_005225 NP_005216.1

E2F1 Antibody, E2F transcription factor 1 Antibody, PRB-binding protein E2F-1 Antibody, RBAP-1 Antibody, RBBP3 Antibody, RBBP-3 Antibody, Transcription factor E2F1 Antibody, RBAP1 Antibody, E2F-1 Antibody, PBR3 Antibody


Specifications

Target
Human E2F1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC (0.1 - 1 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E2F1 antibody was raised against recombinant human E2F-1 protein.
Specificity
Reacts specifically with human E2F-1 protein. It does not cross-react with other related E2F protein. Reactivity with this antibody has been confirmed for human cell lysates.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Immunohistochemistry: 0.1-1.0 ug/ml. Immunoprecipitation: 2-5 ug/IP. Western Blot: 1-2 ug/ml.
Presentation
PBS, pH 7.2, 0.1% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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