Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Rabbit Polyclonal (IgG) to Human E2F1
Western blot, Immunoprecipitation
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Specificity and Use
E2F1 antibody was raised against synthetic peptide: KTPSQEVTSEEENRATDSAT conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 310-329 of Human E2F 1. Percent identity by BLAST analysis: Human (100%); Gorilla, Monkey (95%); Gibbon (85%).
Species cross-reactivity: Cross-reacts with Human.
Suitable for use in Western Blot and Immunoprecipitation.
E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.