Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
E2F-1 in Human Placenta. E2F-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 ug/ml Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue).
Detection of Human E2F1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human skin tissue. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Human E2F1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for E2F1 at approximately 60 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: United States
Date Requested: 1/23/2017