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Anti-E2F1 Antibody (aa264-437) LS-C149926


Wt. Vol. Conc. Price
100 µg - - $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular E2F1 Antibodies

Anti-E2F1 Antibody (clone KH20, KH95) LS-C14959
Mouse Monoclonal [clone KH20, KH95] (IgG) to Human E2F1
Human, Xenopus
Western blot, Immunoprecipitation
Anti-E2F1 Antibody (Internal) IHC-plus™ LS-B6744
Rabbit Polyclonal to Human E2F1
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-E2F1 Antibody (clone 8G9) LS-C169098
Mouse Monoclonal [clone 8G9] (IgG1) to Human E2F1
Human, Mouse
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human E2F1
IHC - Paraffin, Western blot


Human E2F1
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

E2F1 antibody was raised against e. coli-derived recombinant human E2F1 Val264-Phe437 Accession # Q01094.
Detects human E2F1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F2, 3.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About E2F1

Q01094 NM_005225 NP_005216.1

E2F1 Antibody, E2F transcription factor 1 Antibody, PRB-binding protein E2F-1 Antibody, RBAP-1 Antibody, RBBP3 Antibody, RBBP-3 Antibody, Transcription factor E2F1 Antibody, RBAP1 Antibody, E2F-1 Antibody, PBR3 Antibody

E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.


E2F-1 in Human Placenta. E2F-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 ug/ml Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Human E2F1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human skin tissue. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Human E2F1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for E2F1 at approximately 60 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 10/21/2016

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