Bovine Dynein, Cytoplasmic, 74kD Intermediate Chains

Mouse

Bovine, Mouse, Rat, Sheep (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Purified

Unmodified

Bovine brain cytoplasmic dynein.

N-Terminus

Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species cross-reactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.

• ICC (1:50 - 1:100)
• Immunofluorescence
• Western blot (1:1000 - 1:5000)
• Immunoprecipitation

Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10 ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

PBS, pH 7.6, 0.1% Sodium Azide

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.