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Anti-Dynein, Cytoplasmic, 74kD Intermediate Chains Antibody (N-Terminus) LS-C65082

Ordering

Wt. Vol. Conc. Price
100 µg - - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular Dynein, Cytoplasmic, 74kD Intermediate Chains Antibodies

Anti-Dynein, Cytoplasmic, 74kD Intermediate Chains Antibody (N-Terminus) LS-C41545
Mouse Monoclonal (IgG2b) to Bovine Dynein, Cytoplasmic, 74kD Intermediate Chains
Bovine, Mouse, Rat, Sheep, Xenopus, Fish, Drosophila
IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Anti-Dynein, Cytoplasmic, 74kD Intermediate Chains Antibody (N-Terminus) LS-C65082
Mouse Monoclonal (IgG2b) to Bovine Dynein, Cytoplasmic, 74kD Intermediate Chains
Bovine, Mouse, Rat, Sheep
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2b) to Bovine Dynein, Cytoplasmic, 74kD Intermediate Chains
Bovine, Mouse, Rat, Sheep
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Bovine Dynein, Cytoplasmic, 74kD Intermediate Chains
Mouse
Bovine, Mouse, Rat, Sheep (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:50 - 1:100)
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation

Specificity and Use

Bovine brain cytoplasmic dynein.
N-Terminus
Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species cross-reactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.
Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10 ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

Packaging

PBS, pH 7.6, 0.1% sodium azide.
For research use only.

About Dynein, Cytoplasmic, 74kD Intermediate Chains


Requested From: United States
Date Requested: 12/4/2016

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