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Antibodies > DYKDDDDK Tag > LS-C40549
 
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Catalog Number Size Price
LS-C40549-1 1 Each $431 
Antibody

Polyclonal Rabbit DYKDDDDK Tag Antibody (WB) LS‑C40549

Polyclonal Rabbit DYKDDDDK Tag Antibody (WB) LS‑C40549

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Antibody:
DYKDDDDK Tag Rabbit Polyclonal Antibody
Application:
WB
Format:
Unconjugated, Unmodified
Price
Catalog Number
$431
LS-C40549-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rabbit Polyclonal Antibody
Application:
WB
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C40549 is an unconjugated rabbit polyclonal antibody to DYKDDDDK Tag. Validated for WB.
Target
DYKDDDDK Tag
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Specificity
CDYKDDDDK
Applications
  • Western blot (1:200)
Failed Applications
  • Immunoprecipitation
Presentation
TBS, pH 7.4, 5 mg/ml BSA
Reconstitution
Reconstitute with 500 ul deionized water
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Submit a Publication for LS-C40549

Customer Reviews (0)

Submit a review for LS-C40549

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DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Species:
Applications: IHC, Western blot, ELISA
ROBO2 Antibody - Detection limit for recombinant GST tagged ROBO2 is approximately 1 ng/ml as a capture antibody.
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Species: Human
Applications: Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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