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Antibodies > DYKDDDDK Tag > LS-C143065
 
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Catalog Number Size Price
LS-C143065-50 50 µl $416 
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Monoclonal Rat DYKDDDDK Tag Antibody (clone L5, DY650, N‑Terminus, IF, WB) LS‑C143065

Monoclonal Rat DYKDDDDK Tag Antibody (clone L5, DY650, N‑Terminus, IF, WB) LS‑C143065

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Antibody:
DYKDDDDK Tag Rat Monoclonal (N-Terminus) (DY650) (L5) Antibody
Application:
ICC, IF, WB, IP
Format:
DyLight 650, Unmodified
Other formats:
Unconjugated
Price
Catalog Number
$416
LS-C143065-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rat Monoclonal (N-Terminus) (DY650) (L5) Antibody
Application:
ICC, IF, WB, IP
Format:
DyLight 650, Unmodified
Other formats:
Unconjugated

Specifications

Description
DYKDDDDK Tag antibody LS-C143065 is a DY650-conjugated rat monoclonal antibody to DYKDDDDK Tag (N-Terminus). Validated for ICC, IF, IP and WB.
Target
DYKDDDDK Tag
Host
Rat
Clonality
IgG2a Monoclonal
Clone
L5
Conjugations
DyLight 650. Also available Unconjugated.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
N-Terminus DYKDDDDK-tagged extracellular domain of mouse Langerin. Binds to same epitope as Sigma's Anti-FLAG M2 Antibody
Epitope
N-Terminus
Specificity
DYKDDDDK Epitope Tag (L5).
Applications
  • ICC (1:10)
  • Immunofluorescence (1:10)
  • Western blot (1:1000)
  • Immunoprecipitation (1:1000)
  • Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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