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Antibodies > DYKDDDDK Tag > LS-C193485
 
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Catalog Number Size Price
LS-C193485-100 100 µg (1 mg/ml) $754 
DYKDDDDK Tag Antibody - Western Blot of FLAG antibody. 1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with DYKDDDDK-tagged protein vector; unstranfected (1) and transfected (2).

Monoclonal Mouse DYKDDDDK Tag Antibody (clone FG4R, WB) LS‑C193485

Monoclonal Mouse DYKDDDDK Tag Antibody (clone FG4R, WB) LS‑C193485

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Antibody:
DYKDDDDK Tag Mouse Monoclonal (FG4R) Antibody
Application:
WB, IP, ELISA, DB
Format:
Unconjugated, Unmodified
Price
Catalog Number
$754
LS-C193485-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Mouse Monoclonal (FG4R) Antibody
Application:
WB, IP, ELISA, DB
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C193485 is an unconjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for DB, ELISA, IP and WB.
Target
DYKDDDDK Tag
Host
Mouse
Clonality
IgG2b Monoclonal
Clone
FG4R
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.
Specificity
Flag Tag
Applications
  • Western blot (1:1000 - 1:3000)
  • Immunoprecipitation
  • ELISA
  • Dot Blot
Presentation
10 mM PBS, pH 7.2, 0.05% Sodium Azide
Storage
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Customer Reviews (0)

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DYKDDDDK Tag Antibody LS‑C129100
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DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
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DYKDDDDK Tag Antibody LS‑C40549
Species:
Applications: Western blot
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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