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Antibodies > DYKDDDDK Tag > LS-C108816
 
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Catalog Number Size Price
LS-C108816-100 100 µl $403 
DYKDDDDK Tag Antibody - Western blot using Flag mouse monoclonal antibody against two different fusion protein (1), (2) with flag tag.

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 2F11G1, WB) LS‑C108816

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 2F11G1, WB) LS‑C108816

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Note: This antibody replaces LS-C171402
Antibody:
DYKDDDDK Tag Mouse Monoclonal (2F11G1) Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$403
LS-C108816-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Mouse Monoclonal (2F11G1) Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C108816 is an unconjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ELISA and WB.
Target
DYKDDDDK Tag
Host
Mouse
Clonality
IgG2b Monoclonal
Clone
2F11G1
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa(DYKDDDDK), conjugated to KLH.
Specificity
Flag
Applications
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Submit a Publication for LS-C108816

Customer Reviews (0)

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DYKDDDDK Tag Antibody LS‑C129100
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Applications: Western blot, ELISA
DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product
DYKDDDDK Tag Antibody LS‑C40549
Species:
Applications: Western blot
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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