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Anti-DYKDDDDK Tag Antibody (clone 29E4.G7.H11) LS-C154000


Wt. Vol. Conc. Price
100 µg - - $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 29E4.G7.H11] (IgG)
IHC, Western blot, ELISA


IgG Monoclonal [29E4.G7.H11]
Protein A purified


  • IHC
  • Western blot (1:2000 - 1:20000)

Specificity and Use

DYKDDDDK Tag antibody was raised against this antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
The purified antibody is directed against DYKDDDDK and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal antibody detects over-expressed proteins containing DYKDDDDK. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.


Western blot

Western blot
Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Monoclonal Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot.

Western blot

Western blot
Western Blot-Monoclonal Antibody to detect FLAG conjugated proteins. Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 ul. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.

Requested From: United States
Date Requested: 10/25/2016

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