Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > DYKDDDDK Tag > LS-C129097
 
Print Page  
Catalog Number Size Price
LS-C129097-100 100 µg $794 
Antibody

Polyclonal Rabbit DYKDDDDK Tag Antibody (C‑Terminus, WB) LS‑C129097

Polyclonal Rabbit DYKDDDDK Tag Antibody (C‑Terminus, WB) LS‑C129097

Submit Review LSBio Guarantee
Antibody:
DYKDDDDK Tag Rabbit Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Other formats:
Biotin FITC PE
Price
Catalog Number
$794
LS-C129097-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rabbit Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Other formats:
Biotin FITC PE

Specifications

Description
DYKDDDDK Tag antibody LS-C129097 is an unconjugated rabbit polyclonal antibody to DYKDDDDK Tag (C-Terminus). Validated for ELISA and WB.
Target
DYKDDDDK Tag
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-Terminus end.
Epitope
C-Terminus
Specificity
Recognizes DYKDDDDK TAG.
Applications
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:9000 - 1:450000)
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:9000-1:450000. Western Blot: 1:2000-1:10000.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.09% sodium azide
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Submit a Publication for LS-C129097

Customer Reviews (0)

Submit a review for LS-C129097

Featured Products

DYKDDDDK Tag Antibody
DYKDDDDK Tag Antibody (C‑Terminus, clone GST.B6) LS‑C47237
Species:
Applications: Western blot, Immunoprecipitation
Product
DYKDDDDK Tag Antibody LS‑C129100
Species:
Applications: Western blot, ELISA
DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product
DYKDDDDK Tag Antibody LS‑C40549
Species:
Applications: Western blot
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com