Synthetic DYKDDDDK Tag

Rabbit

Synthetic (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Affinity purified

Unmodified

Keyhole limpet hemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-Terminus end.

C-Terminus

Recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. Has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extractshas been shown not to cross-react with any endogenous proteins.

• Western blot (1:1000 - 1:5000)
• ELISA (1:10000 - 1:50000)

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.01% Gentamicin, 1% BSA

Reconstitute with distilled water.

Before reconstitution store at 4°C. After reconstitution store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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