Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Immunogen
Synthetic peptide corresponding to Enterokinase cleavage site, DYKDDDDK (KLH). Residues of cysteine and glycine were used to facilitate coupling at the C-Terminus end.
Specificity
Recognizes DYKDDDDK when fused to either the amino or carboxyl terminus of target proteins. Shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence. Does not cross-react with any endogenous proteins.
Applications
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:10000-1:50000. Western Blot: 1:1000-1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, 0.01% Gentamicin sulfate 1% BSA
Reconstitution
Reconstitute in sterile distilled water.
Storage
May be stored at -20°C lyophilized. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C. Labeled with horseradish peroxidase (HRP). Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee