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Antibodies > DYKDDDDK Tag > LS-C65174
 
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Catalog Number Size Price
LS-C65174-250 250 µg $581 
Antibody

Polyclonal Rabbit DYKDDDDK Tag Antibody (C‑Terminus, IHC, WB) LS‑C65174

Polyclonal Rabbit DYKDDDDK Tag Antibody (C‑Terminus, IHC, WB) LS‑C65174

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Antibody:
DYKDDDDK Tag Rabbit Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$581
LS-C65174-250
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rabbit Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C65174 is an unconjugated rabbit polyclonal antibody to DYKDDDDK Tag (C-Terminus). Validated for ELISA, IHC and WB.
Target
DYKDDDDK Tag
Host
Rabbit
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the C-Terminus end to facilitate coupling.
Epitope
C-Terminus
Specificity
Recognizes the FLAG epitope tag fused to the amino-terminus of targeted proteins. Reacts with all amino-terminal FLAG tagged proteins so far tested. No reactivity is seen against fusion proteins containing the FLAG epitope tag at the carboxy-terminus. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Applications
  • IHC
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Western Blot: 1:1000-1:5000.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Species:
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Species:
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DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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