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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DUSP23 Antibody (Internal) IHC‑plus™ LS‑A6806
Note: This antibody replaces LS-C42458
DUSP23 antibody LS-A6806 is an unconjugated rabbit polyclonal antibody to DUSP23 from human, rat, bat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A6806-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

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Product Description

DUSP23 antibody LS-A6806 is an unconjugated rabbit polyclonal antibody to DUSP23 from human, rat, bat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
About DUSP23
Most of dual-specificity protein phosphatases (DSPs) play an important role in the regulation of mitogenic signal transduction and controlling the cell cycle in response to extracellular stimuli. DUSP23 has been shown to dephosphorylate p44ERK1 but not p38 and p54SAPKbeta in vitro. Reverse transcription-PCR (RT-PCR) revealed that the DUSP23 was expressed in most fetal tissues and two adult tissues: testis and colon. Q9BVJ7 NM_017823 NP_060293.2

DUSP23 Antibody, DUSP25 Antibody, LDP3 Antibody, LDP-3 Antibody, RP11-190A12.1 Antibody, MOSP Antibody, VH1-like phosphatase Z Antibody, VHZ Antibody, VH1-like member Z Antibody


Specifications

Target
Human DUSP23
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Horse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
DUSP23 antibody was raised against synthetic 16 amino acid peptide from internal region of human DUSP23. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Rat, Bovine, Bat, Horse (100%); Gibbon, Mouse, Rabbit (94%); Hamster, Elephant (88%); Opossum (81%).
Blocking Peptide
LS-E29192 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A6806. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human DUSP23. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A6806 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A6806 was determined to be 10 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-DUSP23 antibody LS-A6806 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 7/19/2018

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