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Anti-DUSP1 / MKP1 Antibody (C-Terminus) IHC-plus™ LS-B2469

Note: This antibody replaces LS-C16572

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DUSP1 / MKP1 Antibodies

Anti-DUSP1 / MKP1 Antibody (C-Terminus) IHC-plus™ LS-B2469
Rabbit Polyclonal (IgG) to Human DUSP1 / MKP1
Human, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DUSP1 / MKP1 Antibody (aa355-367) IHC-plus™ LS-B2736
Goat Polyclonal to Human DUSP1 / MKP1
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-DUSP1 / MKP1 Antibody (C-Terminus) LS-C353968
Rabbit Polyclonal to Human DUSP1 / MKP1
Human, Bovine, Rabbit, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Human DUSP1 / MKP1
Human, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human DUSP1 / MKP1
Rabbit
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Monkey, Mouse, Bovine, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 1 µg/ml)

Specificity and Use

DUSP1 / MKP1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%); Monkey, Mouse, Panda, Bovine, Rabbit (93%); Hamster, Pig (87%); Elephant (80%).
C-Terminus
Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.
Immunohistochemistry: LS-B2469 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2469 was determined to be 5 ug/ml. Western Blot: 0.1-1 ug/ml detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 ug/ml).

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About DUSP1 / MKP1

P28562 NM_004417 NP_004408.1

DUSP1 Antibody, 3CH134 Antibody, Dual specificity phosphatase 1 Antibody, ERK-inactivating phosphatase Antibody, HVH1 Antibody, MAP kinase phosphatase 1 Antibody, VH1 Antibody, PTPN10 Antibody, CL100 Antibody, MKP-1 Antibody, MKP1 Antibody

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus.

Immunohistochemistry

Immunohistochemistry
Anti-DUSP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2469 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/3/2016

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