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Anti-DTX1 / Deltex Antibody (aa343-617) LS-C186262

Catalog Size Price
LS-C186262-100 100 µl (1 mg/ml) Unavailable

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20 DTX1 / Deltex Antibodies

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Anti-DTX1 / Deltex Antibody (Internal, AP) LS-C276905
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Anti-DTX1 / Deltex Antibody (Internal, APC) LS-C276907
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Anti-DTX1 / Deltex Antibody (Internal, Biotin) LS-C276908
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Anti-DTX1 / Deltex Antibody (Internal, FITC) LS-C276909
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Anti-DTX1 / Deltex Antibody (Internal, Azide-free, HRP) LS-C276911
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human DTX1 / Deltex
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human DTX1 / Deltex
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

DTX1 / Deltex antibody was raised against recombinant fragment corresponding to a region within amino acids 343 and 617 of Deltex1 (SwissProt Q86Y01). Percent identity by BLAST analysis: Human (100%); Bovine (99%); Mouse (98%); Xenopus (86%).
aa343-617
Human DTX1 / Deltex
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About DTX1 / Deltex

Q86Y01 NM_004416 NP_004407.2

DTX1 Antibody, Deltex homolog 1 (Drosophila) Antibody, Deltex1 Antibody, HDTX1 Antibody, Deltex Antibody, HDx-1 Antibody, Protein deltex-1 Antibody

Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded BT474 xenograft using Deltex1 antibody at 1:500 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of methanol-fixed HeLa using Deltex1 antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. DTX1 / Deltex antibody diluted at 1:1000.

Requested From: 
Date Requested: 12/11/2017

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