Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
DSC3 / Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.
Desmocollin-3 in Mouse Embryo. Desmocollin-3 was detected in immersion fixed frozen sections of mouse embryo (13 d. p. c.) using Sheep Anti-Mouse Desmocollin-3 Antigen Affinity-purified Polyclonal Antibody at 1.7 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis of the developing limb bud.
Detection of Mouse Desmocollin-3 by Western Blot. Western blot shows lysates of mouse embryo (15 d. p. c.) tissue and mouse skin tissue. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Mouse Desmocollin-3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for Desmocollin-3 at approximately 90-110 kD (as indicated). This experiment was conducted under reducing conditions.